CRFT System: Precision Cryopreservation for iPSCs & Organoids

Preserve Your iPSCs & Organoids with Precision. CRFT provides programmable, controlled-rate freezing and thawing without liquid nitrogen ensuring optimal viability for delicate samples.
Applications
iPSCs
  • Optimal cooling between –0.3 to –1.8 °C/min enhances survival
  • Three-zone strategies (fast–slow–fast) fine-tune dehydration and nucleation balance
Organoids
  • Slow, controlled freezing better preserves organoid architecture versus vitrification alone
  • Precise thawing reduces thermal stress and improves post-thaw recovery
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About

Why CRFT for iPSC & Organoid Cryopreservation?

Recent studies highlight that well-controlled freeze/thaw protocols - especially those leveraging programmable controlled-rate systems - achieve >94% viability post-thaw, compared to ~60% with conventional methods

Delicate stem cell and organoid structures need gradual, consistent temperature control during cryopreservation. CRFT offers programmable freeze/thaw profiles that safeguard structural and functional integrity minimising ice damage and maximizing recovery.

Technical highlights:

  • Temperature Range: From -100 °C to +30 °C (Temperature range varies depending on the installed head)
  • Cooling/Thawing Rate: Up to ±10 °C/min with ±0.5 °C accuracy
  • Plate and Sample Adaptability: Handles SBS plates, cryovials, straws via modular heads
  • Data Logging: Continuous monitoring and exportable audit trail

Key Benefits:

  • Liquid-nitrogen-free operation with low running costs and safer handling
  • Real-time data logging for full traceability and reproducibility
  • Interchangeable heads accommodate cryovials, plates, straws, and more
  • Automated-friendly design for seamless integration into workflows
  • Precise control: cooling and thawing at up to ±10 °C/min, ±0.5 °C accuracy, ±1.0 °C uniformity

Products in the range

Video

Specifications

Dimensions (mm) – wdh 345 x 290 x 380 (lid off) / 460 (lid on)
Weight (kg) 5kg
Max plate temp (°C) 30°C
Min plate temp (°C) -100°C*
Temp accuracy +/- 0.5°C
Temp stability +/- 0.1°C
Temp uniformity +/- 1.0°C Ref
Max cooling rate 10°C min/min
Max thawing rate 10°C/min from -100°C
Max temp stabilisation time 5 mins
Plug type UK, EU or US
Power supply 100-240V, 50-60Hz (24V DC unit supply)
Warranty 2 years
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Accessories

H00

Head for 16x 0.5ml straws

H01

Head for 18x0.3ml IMV straws

H02

Head for 55x1.8ml cryovials (0.5ml max fill)

H03

Head for Flat universal plate

H05

Head for 1x SBS microplate

H06

Head for 55x1.8ml deep cryovials (1ml max fill)

CRFTCRYOPEN

Cryopen nucleating tool

CRFTCARTRIDGE

Replacement cartridge for cryopen

CRFT UPS

Un-interruptible Power Supply for the CRFT for a 3 hour cycle in the event of an electrical power failure. 220-240v operation

CAL CRFT

Calibration certificate – single temperature point

CRFTMAINT 1

1 year maintenance agreement includes clean and test report(excludes repair and replacement of faulty parts)

CRFTCOMM

External communications protocol specification for the CRFT

IQOQCRFT

IQOQ booklet for CRFT

PQ CRFT

PQ booklet for CRFT

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Downloads

Software

download
CRFT Software

Product Manuals

download
CRFT Operating Manual

Technical Datasheets

No downloads available

Further Information

download
CRFT Evaluation Report
download
CRFT Flyer
Speak with a product specialist
We can answer your questions and help you find the right products for your needs. Get in touch