Why CRFT for iPSC & Organoids?

Proven outcomes with controlled-rate freezing. Peer-reviewed workflows using a Grant controlled-rate freezer reported ~94% viable hiPSC-derived cardiomyocytes after cryo-recovery, versus ~60% with older approaches; CRFT brings this level of control to bench-scale iPSC and organoid banking. (Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems | Nature Communications)

Reproducible protocols, not folklore. Define cooling, seeding and thaw ramps (including three-zone “fast–slow–fast” freezing profiles) and lock them. Fewer restarts; less user to user variability, optimal cryo recovered patient derived cellular models. (https://pmc.ncbi.nlm.nih.gov/articles/PMC8909336/Ref)

Right tool for 3D biology. Organoid preservation is size and type dependent. Delicate stem cell and organoid structures need gradual, consistent temperature control during cryopreservation. CRFT offers programmable freeze/thaw profiles that safeguard structural and functional integrity minimising ice damage and maximizing recovery. CRFT standardises slow-freeze workflows (CPA equilibration holds; seed at −8 to −12 °C with Cryopen) where slow freezing is preferred/required for some organoids. (https://pmc.ncbi.nlm.nih.gov/articles/PMC8909336/Ref)

Water-bath-free thaw. Program warm-up to standardise recovery and support cleanroom practice; pair with validated dilution steps(e.g., ROCK inhibitor use for iPSCs as required).  (https://pmc.ncbi.nlm.nih.gov/articles/PMC8909336/Ref)

Freeze/thaw without LN₂; store as you prefer. CRFT replaces -80Cfreezer or LN₂  in the process steps; long-term storage is typically in LN₂/vapour or ≤−150 °C per best practice.  (https://pmc.ncbi.nlm.nih.gov/articles/PMC8909336/Ref)

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Technical highlights:

• Temperature Range: From -100 °C to +30 °C (Temperature range varies depending on the installed head)
• Cooling/Thawing Rate: Up to ±10 °C/min with ±0.5 °C accuracy
• Plate and Sample Adaptability: Handles SBS plates, cryovials, straws via modular heads (e.g., 55× 1.8 mL heads; recommended fill volumes 0.5–1.0 mL for control
• Data Logging: real-time logging and exportable audit trail

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Key Benefits:

• Liquid-nitrogen-free operation with low running costs and safer handling
• Precise control: cooling and thawing at up to ±10 °C/min, ±0.5 °C accuracy, ±1.0°C uniformity
• Real-time data logging for full traceability and reproducibility
• Interchangeable heads accommodate cryovials, plates, straws, and more
• True benchtop portability (≈5 kg) for multi-lab deployment
• Automated-friendly design for seamless integration into workflows