EF600M Controlled Rate Freezer

The world's first nitrogen and cryogen free controlled rate freezer has been specifically designed for use in human and animal IVF applications for the cryopreservation of embryos, stem cells and spermatozoa but can also be used for a wide range of freezing applications. The EF600M brings accuracy, precision and reproducibility to biological cryopreservation.

Unlike conventional liquid Nitrogen based controlled rate cooling equipment used in IVF laboratories, the EF600M poses no contamination risk and can be used in cleanrooms and barrier facilities. The EF600M fits neatly and quietly on a bench-top and its performance in terms of cell viability after freezing is comparable or better than standard liquid nitrogen freezers. As alcohol is not used, there is also no potential fire risk. The EF600M will cool down to - 100ºC with straws.

The cooling rate of the EF600M is precisely controlled, ensuring accuracy and reproducibility throughout the freezing profile especially for the important nucleation/seeding phase. This ensures optimal recovery of cells upon thawing. Operation is simple and can be carried out with or without a PC; data can be logged via PC software and cooling profiles are directly displayed. Different cooling profiles are available from a drop down menu and customised profiles can be written. Published trial (1) has demonstrated successful freezing and recovery of embryos, sperm and embryonic stem cells.

Please download the Asymptote Cool Guide to Cryopreservation by Dr J Morris for further information.

(1)RBM Online 13, 421-426, 2006; Cryoletters 27(3), 179-184 (2006)

Features

  • Accurate and reproducible control of cooling rates and sample temperatures
  • Easy to use and samples can be nucleated/seeded in-situ
  • Linear and non-linear cooling profiles
  • Low running costs: estimated at 1% of liquid nitrogen controlled rate freezing
  • Temperature remains at -100ºC at the end of cycle for straw applications until freezer is switched off
  • Uninterruptible Power Supply (UPS): complete cycle run if power fails (supplied as an optional accessory)
  • CE marked
  • Servicing and calibration available
  • 3 year warranty 

Product range

The range includes various models each providing optimum performance for a specific and common vessel, or vessels for the combined heads including: 

EF600M 100 Plate for 16 x 0.5 ml CBS high security straws
EF600M 101 Plate for 18 x 0.3 ml IMV straws
EF600M 102 Plate for 55 x 1.8 ml cryovials (0.5 ml max fill)
EF600M 103 Flat plate for various items/vessels
EF600M 104 Plate for cryocyte bag (available as a "Special" only)
EF600M 105 Plate for 1 x SBS microplate
EF600M 106 Plate for 55 x 1.8 ml cryovials (1.0ml max fill)
EF600M 107 Plate for 10 x 0.5 ml CBS high security straws & 12 x 1.8 ml cryovials (0.5 ml max fill)
EF600M 108 Plate for 12 x 0.3 ml IMV straws & 12 x 1.8 ml cryovials (0.5 ml max fill)

Applications

The EF600M is highly versatile and can be used for the cryopreservation of a wide range of samples in cryovials, straws, bags, microplates and Matrix-96-well block plates including:

  • Human and veterinary IVF
  • Transgenic embryos
  • Stem cells
  • Clinical and research samples, e.g. lymphocytes and tissue cells lines in conventional cryovials
  • Various mammalian cells including cardiomyocytes, adipose, liver and muscle
  • Cord blood derived stem cells in typical cryocyte bags
  • Adherent cells and stem cells in microplates plates
  • Cell suspensions in numbered/barcoded arrays for archiving and storage
  • Robotic integration - the EF600M has also successfully been integrated into robotic systems, supporting biorepository and biobank applications